Introduction of CSY-E96H aflatoxin rapid detector
The principle of aflatoxin rapid detector: using the principle of solid phase enzyme-linked immunosorbent assay, using indirect competitive ELISA. After coating the microplate with antigen, adding an aflatoxin standard or sample for immunoreaction, the antibody not bound to the coated antigen is washed away; then the enzyme-labeled anti-mouse IgG antibody is added to incubate, and the substrate is added for color development. After termination of the stop solution, the OD value was measured at 450 nm using a CSY-E96H aflatoxin rapid detector .
Reagents and materials required for rapid detection of experimental procedures: microplates are coated with AFB1-BSA; 5 concentrations of AFB1 standard solution (1 mL) can be used directly;
Standard 1:0ng/mL can be used directly;
Standard 2: 1.0ng/mL can be used directly;
Standard 3: 2.0ng/mL can be used directly;
Standard 4: 5.0 ng / mL can be used directly;
Standard 5: 10.0 ng / mL can be used directly;
AFS monoclonal antibody solution (6mL) can be used directly;
The enzyme standard (12mL) can be used directly;
Color developing solution (12mL);
Stop solution (6mL);
Concentrated washing solution (30mL);
Blank control (6ml) microplate CSY-E96H aflatoxin rapid detector (including 450nm): for quantitative analysis; oscillator, pulverizer, micropipette, measuring cylinder, funnel and volumetric flask, fast qualitative filter paper; reagent: Sodium chloride (analytical grade), methanol (analytical grade), deionized water or distilled water
Note on inspection operation: Aflatoxin should be included in the standard solution and special care should be taken. The used glass container and aflatoxin solution are preferably soaked overnight with a pH 7 hypochlorous acid solution (10% v/v). The stop solution is 0.5N sulfuric acid to avoid contact with the skin. Do not exchange reagents from different lot numbers.
Aflatoxin test sample pretreatment: samples should be stored in a cool and dark place and stored in cold storage. Take 5g of representative sample, add 1.25g sodium chloride and 25mL 70% methanol-water, shake vigorously for 3 minutes. Filtration with fast qualitative filter paper; take 1 mL of filtrate and dilute 10 times with 1% sodium chloride solution; take 50 μL of the diluted solution for analysis; increase the sample amount as needed, but the amount of methanol solution should also increase accordingly.
Inspection test precautions and measurement procedures: All reagents should be returned to 2-8 °C immediately after each sample addition, and the operation time of each step should be controlled within 5 min. The incubation should be protected from light. Assay Procedure 1. Insert the required number of slats into the microwell rack and record the sample and standard position.
2. Add 50 mL of standard or processed sample to the wells and use a new tip for each standard and sample.
3. Add 50 mL of anti-aflatoxin monoclonal antibody solution to each well (note that the pipette tip does not touch the liquid placed in the well to avoid cross-contamination). Add a blank in the appropriate position. 100 mL, incubated at 37 ° C for 90 min.
4. Remove the liquid from the well and wash the microplate with the wash solution for 3 min × 3 times. The last time should be tapped on the absorbent paper to completely remove the liquid from the well.
5. Add 100 mL of the enzyme standard to each well and incubate at 37 ° C for 60 min.
6. Remove the liquid from the well and wash the microplate with the wash solution for 3 min × 3 times. The last time should be tapped on the absorbent paper to completely remove the liquid from the well.
7. Add 100 mL (2 drops) of the color solution to each well and incubate at 37 °C for 10 min -12 min.
8. Add 50 mL (1 drop) of stop solution to each well and immediately measure the absorbance value (OD value) at a wavelength of 450 nm using a CSY-E96H aflatoxin rapid tester.
Freeze-Dried Fruit,Freeze Dried Organic Fruit,Organic Freeze Dried Fruit,Freeze-Dried Fruit Powder
Shaanxi HuiKe Botanical Development Co.,Ltd , https://www.oasis-hk.com