Chinese name: human unphosphorylated insulin-like growth factor binding protein-1 ELISA kit
English name: Insulin-like growth factor binding protein 1 ELISA Kit
Precautions
1. When mixing protein solutions, try to be gentle and avoid foaming.
2. The washing process is very important, and insufficient washing can cause false positives.
3. It is best to control the loading time within 5 minutes. If the number of specimens is large, it is recommended to use a gun.
4. Please make a standard curve at the same time of each measurement, it is best to make a double hole.
5. If the content of the substance to be tested in the specimen is too high, please dilute it before measuring it. When calculating, multiply by the dilution factor.
6. When preparing the standard and test solution working solution, please prepare with the corresponding dilution solution, not to be confused.
7. Keep the substrate away from light.
8. Do not replace the reagents in the kit with reagents from other manufacturers.
Features:
1. Strong specificity. The immune response between the antigen and the antibody is a specific reaction, and the immunoenzyme technology is based on an immune reaction. The detected object is an antigen (or an antibody), and the antibody used has no immunological reaction characteristics with the common antibody except the labeled enzyme. How big is the difference.
2. High sensitivity. Since the antibody is bound to the enzyme, the binding reaction between the antigen and the antibody is revealed by the color reaction of the enzyme with the substrate, which greatly improves the sensitivity of the detection, and the detection level is close to the radioimmunoassay.
3. The sample is easy to store. Most of the colored products revealed by the enzymatic reaction are relatively stable, which is beneficial to the preservation of the sample.
4. The results are easy to observe. The test results can be observed with the naked eye, and can be observed with a microscope, or can be measured by a spectrophotometer, and can also be observed with a microscope. This is because some enzyme reaction products can change the electron density, thereby causing the display of the detected object.
5. Can be quantitatively determined. The antigenic substance in the solution is subjected to colorimetric measurement using an enzyme-free adsorption technique, and can be quantified depending on the change in optical density value. It is expected that a cell spectrophotometer can be used for quantitative determination of antigens in tissues by immunoenzymatic techniques.
6. The sample can be measured on a large scale. If thousands of samples need to be tested, the enzyme technology can be completed in a short period of time.
This product is provided by Shanghai Qiaoyu Biotechnology. For more details about human ELISA kit, please contact customer service staff in time. We will provide you with one-on-one answers and perfect after-sales service.
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