Lips 50 (E50) milk antibiotic detection reagent operation specification

one. Steps

1. Reagents hole cutting with a knife, a first longitudinal cut (8), and then a transverse cut or two, three ...... (cut along the slit between two holes).

2. Take out the cut test hole and mark the corresponding sample ( you can mark the number with a fine pen on the side ) to uncover the sealed aluminum foil.

3. Each sample (50 μl ) was aspirated in the order of the sample using a sampling gun and added to the corresponding single well. Note: Each sample head corresponds to one sample and the used sample head is thrown away.

4 . After all the samples have been added, the corresponding sealing strips are cut to seal the test single holes. (Corresponding to the sealing strip already cut single well, simply take Shredded, detecting an amount of less than 8, and then clipping the application of scissors).

5 . The sealed single well is placed in a water bath or a constant temperature incubator for cultivation, and the culture time is about 2 hours and 30 minutes.

6 . The test after the culture was taken out, the color of the single hole was observed from the side, and the detection result was recorded.

Note: Interpretation of results

Yellow to light yellow green including green for negative result range

Cyan to purple is a positive result range

7. In order to ensure the accuracy of each test and the reason for finding the wrong result, it is recommended to make a negative control for each test ( with no anti-milk as a negative control ) , and if possible, a positive control (10 ppb penicillin sample as positive) Control, in order to ensure that the reagents are normal ) .

two. Reagent preservation

1 . Storage temperature: 4-12 °C ( General refrigerator storage cabinet can meet the storage conditions )

2 . Protect from light: This product is sensitive to sunlight. Generally, do not expose it to sunlight for a long time, or under the light.

Note: The reagents can not be stored frozen. The internal components of the frozen reagents will be destroyed. The reagents and samples should be returned to room temperature before the detection operation can be started. However, the remaining detection holes should be put back into the refrigerator immediately to avoid the effects of repeated multiple times. The performance of the reagents.

three. be careful

1 . The culture tool can be used in a constant temperature water bath or a biochemical incubator. The temperature must be strictly controlled at 65 °C ± 1 °C. Exceeding this temperature condition, the test results will be affected, and serious false positive and false negative results will appear.

2. The unused reagents on each plate must be put back into the box and kept in the refrigerator. Be sure to use the original plastic bag to continue sealing. In addition, do not put the small holes that have been torn open or the small holes that accidentally break the aluminum paper into the refrigerator for use. If you accidentally break the small holes, seal them with adhesive paper and use them in the fastest time. Finish.

3. When using a constant temperature water bath, seal the test holes after the sample is added with the supplied adhesive paper to avoid water ingress due to heat. In addition, place the reagent well inside the frame plate and slowly place it in the water bath to float on the water. Do not put the whole frame plate below the water surface, which will make the adhesive paper sticky or reduce, so that water vapor or water enters the small hole, and the detection result will be deviated.

4. The use of the sample gun, the sample gun has two gears, whichever is the first one, gently press down until it needs to be pressed down with a large force, this is a file. It is required to take a milk sample in the first block and a milk sample in the second gear, so as to ensure that the sample loading amount reaches 50ul . The capacity of 50ul is probably about 2mm of milk sample , or about 2 drops of semi-added gun. If it exceeds this height, it is likely that the gun is wrong or the gun is not accurate.

5. Regularly measure the temperature of the water bath ( preferably for 30 minutes continuously to determine if the temperature can still be within the normal working range ) to ensure the accuracy of the experiment. If you use a constant temperature incubator, you need to confirm the temperature and cannot exceed the specified range. The dedicated FX heater does not require temperature confirmation.

6. The milk that has just been squeezed cannot be directly tested. It is necessary to put the milk in the freezer of the refrigerator for 1-2 hours before starting the test. The hot milk that has just been squeezed contains many microbial-inhibiting components, which affects the false positive of the test results. .

7 . The pH of the sample is required to be the same as the pH of the raw milk , which is about 6.6 . If the pH is too high or too low, it may cause erroneous results. If the PH value is too high, it will lead to false positive results. If the PH value is too low, it will lead to false negative results. So measure the pH of the sample before each test .

8. If the milk sample penetrates into the bottom of the small hole (a thick white layer at the bottom ) or a white milk sample on the side of the small hole, it proves that the reagent has experienced a very low temperature ( temperature below 2 °C). ) , for this detection hole, it is recommended to do another test to confirm the result.

ECLIPSE50, referred to as E50, was introduced into China by Spain and was incorporated into the dairy regulations as the second national standard. According to the fact that the previous regulations were difficult or long-term changes, it can be assumed that it will be a long-term enforcement regulation. E50 uses a microbial inhibition method. The bacillus in the reagent grows fastest at 65.0 °C. Other temperatures will reduce the growth rate of the bacillus. If the experimenter uses a water bath and incubator whose temperature cannot be constant, the customer is at the most The results cannot be interpreted at the time of good training.