The alkaline phosphatase (ALP) assay kit is intended for the accurate and direct quantitative measurement of ALP activity in human plasma. ALP measurement is useful in the diagnosis of liver, bile diseases and bone disorders.
Test principle
4-Nitrophenyl Phosphate (4-NPP) can be dephosphorylated by ALP and converted into 4- Nitrophenol (4-NP), which turns yellow due to it quinoid structure formed in alkaline environment. The unit activity of ALP can be calculated from the increasing rate of absorbance detected at a wavelength of 405 nm.
Product Features
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Linear deviation ≤ ± 10.00%
Technical Parameters
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Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
Main wavelength | 405 nm | Secondary wavelength | 480 nm (Optional) |
Method | Velocity method | Reaction direction | Positive |
Sample | 4 μL | R1 | 200 μL |
R2 | 50 μL | Delay time | 50 s |
Reaction time | 180 s | Â | Â |
Packing Specifications
R1: 2×40 mL, R2: 1×20 mL
R1: 4×60 mL, R2: 4×15 mL
R1: 1×40 mL, R2: 1×10 mL (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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The alkaline phosphatase (ALP) assay kit is intended for the accurate and direct quantitative measurement of ALP activity in human plasma. ALP measurement is useful in the diagnosis of liver, bile diseases and bone disorders.
Test principle
4-Nitrophenyl Phosphate (4-NPP) can be dephosphorylated by ALP and converted into 4- Nitrophenol (4-NP), which turns yellow due to it quinoid structure formed in alkaline environment. The unit activity of ALP can be calculated from the increasing rate of absorbance detected at a wavelength of 405 nm.
Product Features
Â
Linear deviation ≤ ± 10.00%
Technical Parameters
Â
Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
Main wavelength | 405 nm | Secondary wavelength | 480 nm (Optional) |
Method | Velocity method | Reaction direction | Positive |
Sample | 4 μL | R1 | 200 μL |
R2 | 50 μL | Delay time | 50 s |
Reaction time | 180 s | Â | Â |
Packing Specifications
R1: 2×40 mL, R2: 1×20 mL
R1: 4×60 mL, R2: 4×15 mL
R1: 1×40 mL, R2: 1×10 mL (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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The small tube is a kind of squid, which is characterized by a small body and a long and slender body, also called the squid.
Squid meat is more delicious than cuttlefish. The head and torso are longer and narrower than that of a cuttlefish, especially the end of the torso are very similar to the spearhead of a javelin, so it is called a squid. Octopus, also known as octopus, belongs to the order of the eight arms.
Both octopuses and cuttlefish have ink sacs that can release the ink. The biggest difference is that the octopus has only 8 wrists, and the suction cup on the wrist does not have a handle or toothed ring.
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