Natural product separation
The basis of pharmacodynamics in traditional Chinese medicine is the compound in which Chinese medicine enters the body. The research on the chemical constituents of traditional Chinese medicine requires separation and structural identification of natural products in traditional Chinese medicine. Moreover, there are regional differences in the composition and content of natural products in traditional Chinese medicine. The quality identification of medicinal materials is only difficult to satisfy from the morphological form. Therefore, it is necessary to clarify from the chemical composition and provide a scientific basis for establishing quality standards for medicinal materials. Separation and structural identification of certain natural products in traditional Chinese medicine also provide a material basis for drug discovery based on the dominant structural backbone or unit of natural products.
EYELA products---natural product separation case
Firstly, a certain amount of medicinal material powder was placed in a round bottom flask, a certain amount of ethanol was added, reflux extraction was carried out for 2 h, and the mixture was filtered and refluxed. The combined filtrate was concentrated by EYELA rotary evaporator (water bath temperature 40 ° C, vacuum 20 hPa). The extract is obtained, and the recovered solvent ethanol is reused.
The extract was applied to a macroporous resin column, eluted successively with water, (30%-50%) aqueous ethanol solution, and the eluate was concentrated by EYELA rotary evaporator (water bath temperature 40 ° C, vacuum 20 hPa). The extract is applied to the adsorption silica gel column, the eluent is eluted to obtain a silica gel column containing different components, and a silica gel column is eluted with an alcohol solvent to obtain an eluate which is concentrated by rotary distillation and The concentrate was dissolved and applied to a C18 silica gel column, and the alcohol solvent was eluted to obtain an eluate containing the target component.
Freeze drying
The eluate was diluted in 5-10 volumes of purified water in a watch glass, pre-frozen at -20 °C for 2 h, and lyophilized at a cold trap temperature of -45 ° C and a vacuum of 10 Pa (EYELA FDU-1200). When the sample quality does not change, the freeze-drying is stopped, and the vacuum pump and the refrigerator are sequentially turned off to obtain a lyophilized sample.
EYELA concentration and freeze-drying equipment plays an important role in the separation of natural products of this traditional Chinese medicine, and provides reliable means of concentration and freeze-drying to provide a guarantee for the structural identification of natural products of subsequent Chinese medicines.
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