Nonsmall-cell lung carcinomas (NSCLC) account for about 80% of all lung cancers, and most patients are already in the advanced stage, with a 5-year survival rate. For NSCLC, targeted therapy is an ideal treatment for the clinic. Epidermal growth factor receptor (EGFR) is currently an important target for the treatment of NSCLC, but related target drugs such as EGFR tyrosine kinase inhibitors may lead to drug resistance mutations in tumor cells. Therefore, real-time monitoring of mutations is necessary during the treatment process. Traditional biopsy methods cannot be used for real-time monitoring, and circulating tumor cells (CTCs), which can be used as substitutes for tumor tissue, have the potential to achieve real-time monitoring.
Regarding CTC cell assays, the CellSearch system is the only US FDA-approved CTC counting system, but it is still difficult to use for CTC analysis in NSCLC patients. Recently, Japanese scientists published the "Isolation and molecular analysis of circulating tumor cells from lung cancer patients using a microfluidic chip type cell sorter" in the Journal of Cancer Science in the Journal of Cancer Science by means of the On-chip Sort flow sorter. . Studies have shown that the use of On-chip Sort is an effective method for detecting and capturing CTC cells in NSCLC patients.
First, the researchers mixed different numbers of various tumor cell lines into the blood and detected and isolated CTC cells using On-chip Sort. In the isolated CTC cells, the success rate of EGFR mutation detection was 100%. The researchers then collected peripheral blood from 30 NSCLC patients and used On-chip Sort and CellSearch to calculate the number of CTC cells in a blind manner. The results showed that 22 (73.3%) of the 30 patients were positive for CTC using On-chip Sort, but only 9 (30%) were positive for CTC using the CellSearch system. On-chip Sort was also confirmed as positive for the 9 patients who were positive for CellSearch. It is indicated that On-chip Sort has higher sensitivity than CellSearch system in CSC cell detection of NSCLC patients. Some CTC cells that cannot be detected by CellSearch can be detected by On-chip Sort.
In addition, the researchers used fluorescence microscopy to observe the cells captured by On-chip Sort sorting (these cells have been labeled with fluorescent antibodies that target tumor cell markers prior to sorting). The results showed that the sorted cells were bound to the tumor marker fluorescent antibody, indicating that the CTC cells were sorted.
to sum up:
On-chip Sort can accurately separate CTC cells from NSCLC patients and can be used for subsequent tumor cell DNA detection to achieve real-time monitoring of EGFR mutations. In addition, On-chip Sort-based CTC cell analysis provides unique advantages in cell morphology analysis, immunocytochemistry, and fluorescence in situ hybridization assays. Most importantly, this method can be used to assess changes in EGFR protein expression levels and has great clinical potential in the diagnosis of CTC cells in NSCLC patients.
About On-chip Sort Flow Sorter
Technological innovation
â— Innovation: using patented microfluidic technology;
â— No damage: low pressure gas promotes sorting;
â— Zero pollution: the disposable chip is completely enclosed and has no clinical pollution;
â— Unrestricted: There is no requirement for sheath fluid during sorting (sea water, culture fluid, oil, etc. can be used);
Application field
â— CTC sorting and identification;
â— Nerve cell sorting and identification;
â— Determination of bacteria, microorganisms, molds and viability;
â— sperm sorting;
â— Blood cell sorting and identification;
â— Stem cell research (iPSES cells);
â— drug mechanism research;
â— ......
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