The world's first robotic "fencing" cloned pig was born in Tianjin
July 5, 2017 Source: China News Network
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];Recently, after a long wait of more than two months, a special "parental identification" report was released. 13 cloned piglets were not related to the "surrogate" mother, and only had a "parental relationship" with the donor cells. This is medically proven that the world's first robotic somatic cell cloned pig was born in Tianjin, China.
After 110 days of gestation, on April 26th and 29th, two ordinary "surrogate" sows successfully produced 13 healthy purebred small Landrace pigs. Compared with the previous "manual operation" cloning technology, the robot's automated "fencing" is less forceful, less damage to cells, and higher precision operation. The key indicator of successful cytoplasmic cloning technology is the blastocyst rate. 10% increased to 20%. This data surprised the global peers.
The "robot operating somatic cell clone pig" research is from the interdisciplinary research team led by Professor Zhao Xin from Nankai University Robotics Institute. Tianjin Animal Husbandry and Veterinary Research Institute is the main cooperation unit. The "Micro-Operation Robot System for Biomedical Engineering" developed by Nankai University Robotics Institute has won the second prize of the 2002 National Technology Invention. The birth of this cloned pig is another major breakthrough in the technology after 15 years.
Somatic cell cloning is one of the classic methods for improving biological species. It removes the nuclei of the common cultivar oocytes and injects them into the somatic cells of the elite variety. The advantage of this method is that the offspring obtained must be excellent varieties. However, the success rate is extremely low, which has become a bottleneck in the development of somatic cell cloning technology.
"As one of the most complicated micro-operations, somatic cell cloning technology urgently needs to be robotized. It is the real face that we face by robotic somatic cell nuclear transfer operation, improving the success rate of subsequent cell culture, and thus improving the success rate of whole somatic cell cloning technology. Challenge." Zhao Xin said.
In response to the above problems, the research team of Nankai University has carried out scientific research on the development needs of life sciences, and developed in-situ microscopic analysis and operation instruments with visual, minimally invasive, fixed-point and quantitative functions, integrating detection, analysis and operation. This instrument is used to realize the robotic nuclear transfer process.
According to reports, the key difficulty of somatic cell cloning technology based on robot micro-operation is how to minimize damage to cells. The Nankai University team designed and implemented a robotic nuclear transfer process, including oocyte plucking to find polar bodies, enucleation and somatic cell injection.
By analyzing the cell stress during the process of micro-operating tools and cell contact, the researchers realized the cell movement and enucleation based on the minimum force, which ensured that the cell stress during the nuclear transfer operation was minimal. “Manually manipulate the cells, the maximum deformation of the cells is 30 to 40 microns. After calculation, the maximum deformation of the robot operating cells is reduced to 10 to 15 microns. If the previous strength is 'a big punch', then it is now Push '.†Zhao Xin introduced that the micro-operating system uses the operation based on the balanced pressure model, which also makes the process of extracting the nucleus more "controllable" and "gentle".
The experimental results show that the cell force-based method based on the minimum force significantly reduces the damage to the cells; the subsequent cell culture shows that the cell development rate is significantly higher than the manual operation based on the minimum force-based cell enucleation operation.
“The most exciting thing is that in vitro experiments, we used this micro-operating platform to carry out nuclear transfer of 53 oocytes, 11 of which were developed into blastocysts that marked the successful cloning, with a development rate of 21%. The blastocyst rate of somatic cell clones has remained at around 10%," Zhao Xin said.
In order to make this technology "flowering result", the research team of Nankai University applied the nuclear transfer method to the whole cloned pig process, and completed thousands of nuclear transfer operations to verify the effectiveness of the method. At the beginning of January 2017, 510 nuclear transfer operations were completed in four batches, and the 510 cloned embryos were transplanted into six sows. In the end, the two sows were successfully conceived, and they produced 7 and 6 heads, respectively, on April 26 and 29, for a total of 13 healthy cloned pigs.
“Our research is the first to guide micromanipulation from the perspective of cell development, and to link the micromanipulation process with cell development results through cell stress. The promotion of this method can further enhance the contribution of micromanipulation technology to the overall biological process. Foreseeable, it has a good application prospect in the fields of assisted reproduction, animal and plant variety improvement, mass medical and livestock production," Zhao Xin said.
It is understood that the project has won the national major scientific research instrument development project, the advanced manufacturing field of the "863" program, and the special science and technology special support of Tianjin intelligent robot. (Zhang Daozheng Wu Junhui)
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