First, the appearance of serum, the first contact with the appearance of serum, for those who lack experience in cell culture, the appearance of the judgment is particularly important.
1. Color According to the different hemoglobin content, fetal bovine serum can show yellow or red. The serum standard for cell culture in China is no more than 20mg/dl. In fact, the level of hemoglobin has no direct effect on serum. The significance is to reflect the rigorous standardization of the blood collection process, good operation can reduce the hemolysis of serum.
The blood collection points of domestic serum are very scattered, and most of them are collected by the butcher immediately after blood collection, so there is very little hemolysis, showing a bright waxy yellow. Of course, domestic serum rarely has fetal calf serum in the standard sense.
Imported fetal bovine serum is more or less hemolyzed, because the true fetal bovine serum has poor coagulation function and red blood cells are easily broken.
In general, domestically produced serum exhibits sallow. Imported serum, the best quality of the yellow, reddish, such as Gibco16000, almost appeared orange or bright red. Of course, heat-inactivated serum or improperly preserved serum, due to the destruction of hemoglobin, will appear dark red or even brown.
2. Precipitation Many serum customers will find that the imported serum has a precipitate, which is suspected of the quality of the serum. This is unfounded.
The precipitation in serum is produced by the aggregation of fibrin, which has no effect on the serum quality. There are many reasons for the precipitation, which is closely related to the processing and production methods of the manufacturers.
Domestically produced serum, mostly from the north, due to low prices, the preservation environment is not good, from blood collection, freezing, transportation to production will undergo multiple freeze-thaw cycles, each freeze-thaw process will precipitate part of fibrin precipitation, so serum After the finished product is filtered, it looks more "clean" and there is very little precipitation.
Imported fetal bovine serum is rarely frozen and thawed before filtration. The finished product is also clear, but over time, a large amount of fibrin in the serum will precipitate and form a precipitate. Of course, the imported newborn bovine serum is generally about 2 weeks old, and the various protein contents in the serum are obviously high. After the production, the serum may be cloudy or even precipitated.
Of course, the serum that is purchased is frozen, and needs to be melted when used. The melting process is preferably carried out slowly at a lower temperature, otherwise the precipitation is relatively more, although the precipitation does not directly affect the quality of the serum, but the cells Microscopic observation has certain obstacles.
3, clarity of imported fetal bovine serum due to low content of protein and lipids, the performance is thin, light, and domestic serum, because the vast majority of newborn bovine serum, relatively more viscous, slightly darker color, this is People who are initially exposed to serum are difficult to judge, but comparing the two can be easily distinguished.
Second, the main technical indicators The current domestic serum quality standards are the 2005 edition of the "People's Republic of China Pharmacopoeia", the main indicators have the following:
Third, the blood source of blood in the blood source market is mainly divided into China, North America, South America and Australia.
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