CBP-labeled proteins are capable of being heterologously captured by calmodulin resin in low-concentration calcium buffer and can be eluted by 2 mM EGTA in a neutral environment, which is comparable to the 6-histidine affinity tag purification system. The conditions should be much milder. The 4-kDa-sized CBP tag has a very small effect on protein molecules compared to the 26-kDa GST tag. The inclusion of a thrombin or enterokinase listing site on the expressed protein molecule allows for easy removal of the CBP tag. (CBP Tag : KRRWKKNFIAVSAANRFKKISSSGAL)
How to choose the right CBP tag antibody?
When choosing CBP-labeled antibodies, we mainly rely on our own needs, especially the needs of experimental applications. In addition, the more types of applications after an antibody is detected, the greater the choice in use; while the monoclonal antibodies from mouse sources generally have better specificity, stability, and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high-potency 100μl CBP antibody (if the dilution ratio of WB is 1:5000, the final use solution is equivalent to 500ml), 1ml of CBP antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml).
| Product name and description | Item number | product manual | |
| Anti-CBP Tag Monoclonal Antibody(12H5) | A02190 | Details | |
Why recommend the Abbkine brand CBP tag antibody (clone 12H5 )?
At present, the CBP-labeled antibodies available on the market are domestically packaged or produced. The domestically packaged CBP-labeled antibodies have insufficient quality stability, and after being packaged, their potency is also lost. Some CBP-labeled antibodies originally produced in domestic laboratories have good detection activity and are difficult to control in batch stability. However, only a small amount of domestically produced antibodies are polyclonal antibodies. As for the imported brands that supply CBP-labeled antibodies, there are not many imported brands. Only Abcam, Sigma and other brands can provide CBP-labeled antibodies, but they are far less expensive than Abbkine products.
"Comprehensive comparison of CBP tag antibodies"
| Brand | Item number | App types | Price / specification |
| Abbkine | A02110 | WB: (1:5000-1:10000) | 605/50ul 1800/200ul 6020/1ml |
| Abcam | Ab119488 | WB: (1:250-1:2000) | 4076/100ul |
Therefore, we recommend Abbkine's Anti-CBP Tag Monoclonal Antibody (12H5). Like Abcam, Abbkine's CBP antibody immunogen is a synthetic CBP peptide. After continuous screening and optimization, the best cell line is finally obtained (12H5). ). As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. This antibody can be used for WB.
Antibody verification results (A, 1:5000 dilution; B, 1:10000 dilution)
| Product name and description | Item number | product manual | |
| Anti-CBP Tag Monoclonal Antibody(12H5) | A02190 | Details | |
Abbkine also offers 1ml large packaging and larger custom-made CBP antibodies, making it ideal for routine testing of CBP immunoassays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during use, please contact Abbkine local agent or Aimei Technology Co., Ltd. directly.
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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