BIOG DNA Saliva Kit (Saliva DNA Extraction Kit) Instruction Manual

Transportation conditions : normal temperature transportation

Item: 51043: 50 reactions

51044: 100 reactions

product description

The BIOG DNA Saliva Kit is a kit developed by Changzhou Baidai Biotechnology Co., Ltd. for the extraction of saliva. The BIOG DNA Saliva Kit uses the latest high-quality imported ion membranes, and the lysate and eluent are optimized several times to efficiently separate DNA. Compared with other brands, the extracted DNA has higher yield, higher purity, and maximizes the removal of impurities such as proteins, pigments, lipids, etc., and can be directly applied to PCR, real-time PCR and various enzyme digestion tests. .

Features

â—ŽThe extracted DNA has high purity and no inhibitor, and the A260/A280 is 1.7-1.9;

â—Ž high yield, more DNA extracted from the same sample size;

â—Ž can be used for DNA extraction in saliva samples, and the extracted DNA can be used for nucleic acid detection;

â—Ž It is free of toxic solvents such as phenol and chloroform, safe and non-toxic.

Kit composition

Steps

1. Please prepare yourself: anhydrous ethanol, normal saline, centrifuge tube.

2. Remove the precipitate and wash solution as follows:

a) Washing solution A: 21 mL was added to 9 mL of absolute ethanol; 42 mL was added to 18 mL of absolute ethanol.

b) Washing solution B: 9 mL was added to 21 mL of absolute ethanol; 18 mL was added to 42 mL of absolute ethanol.

c) If the prepared precipitate and washing solution are precipitated, they can be dissolved at 37 ° C, shaken and used.

3. Take 300 μL of saliva into a centrifuge tube, add 200 μL of lysate, 20 μL of digestive juice and 4 μL of DNA Carrier, mix by shaking, and bath at 56 ° C for 10 minutes.

4. Add 800 μL of absolute ethanol and mix gently by inversion. If there is a translucent suspension, it will not affect the DNA extraction and subsequent experiments.

5. Place the adsorption column into the collection tube, transfer 650 μL of the above solution into the adsorption column, let stand for 2 minutes, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube;

5. Return the column to the collection tube and transfer the remaining 674 μL of the solution to the column. Repeat step 5.

6. Put the adsorption column back into the collection tube, add 500 μL of washing solution A to the adsorption column, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube.

7. Place the adsorption column back into the collection tube, add 500 μL of Washing Solution B to the adsorption column, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube.

8. Place the column back into the collection tube and centrifuge at 12,000 rpm for 4 min at 4 ° C to remove any remaining wash solution.

9. Add the adsorption column, place it in a new 1.5 mL centrifuge tube, add 30-60 μL of the eluate, let stand for 3 min, centrifuge at 12,000 rpm for 4 min at 2 °C, and collect the DNA solution. The extracted DNA can be used in the next experiment or at -20 °C.

Precautions:

Lysates, precipitates, and washings contain irritating chemicals. Take precautions during operation to avoid direct contact with the skin and prevent inhalation of nose and mouth. If you accidentally get into skin or eyes, rinse immediately with water or saline. Seek medical attention if necessary.

Changzhou Baidai Biotechnology Co., Ltd.

Address: New Oriental Logistics Park, No. 51 Dongfang East Road, Changzhou Tel: 0519-88050392 Fax: 0519-83382790

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