Hygromycin B is an aminoglycoside antibiotic produced by the metabolism of Streptomyces hygroscopicus, which kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Hygromycin B inhibits protein synthesis by interfering with 70S ribosome translocation and inducing misreading of mRNA templates. It is currently used to screen and maintain cultured prokaryotic or eukaryotic cells containing a hygromycin resistance gene.
Application of hygromycin B:
1) screening and maintaining culture of prokaryotic or eukaryotic cells (plant cells and mammalian cells) stably transfected with the Hph+ vector;
2) Due to the difference in mode of action, it is often used in combination with G418 (GeneticinTM), ZeocinTM and blasticidin S to screen cell lines stably transfected with two different vectors.
3) antiviral agents, because hygromycin B selectively infiltrates into cells that enhance permeability due to viral infection, and has the effect of inhibiting translation;
4) Adding animal feed as an anthelmintic; the effective concentration of mammalian cells is usually selected between 50 μg/ml and 1 mg/ml; for plant cells, the effective concentration is 20–200 μg/ml; for bacteria, the effective concentration is 20–200 μg/ml; for fungi, the effective concentration is 200 μg–1 mg/ml.
Chemical properties:
Hygromycin B is a yellowish brown amorphous powder with a special odor and a melting point of 160-180 °C. It is a weak base, easily soluble in water, methanol, and ethanol, and easily forms salts with many organic and inorganic acids.
Storage method: Store the solution directly at 2 ° C ~ 8 ° C, at least for 1 year.
Beijing Suo Laibao Technology Co., Ltd. has the spot product Hygromycin B, the product number is H8080, which is a product of Germany. The solubility is 100mg/ml and the titer is 1100 units/mg. It is used for biochemical experiments. Specific antibiotics used in transfection; hygromycin B phosphotransferase inactivates hygromycin B; cells transfected with hph gene are resistant to hygromycin, and the transfected cells stably or temporarily express the gene.
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