Dolomite microfluidic chip successfully used for high-throughput single-cell DNA/RNA sequencing

With the development of modern biology, the research of cell populations can no longer meet the needs of scientific research. Single-cell sequencing solves the problem of cell heterogeneity that cannot be solved by sequencing tissue samples or when samples are small, and provides a new direction for scientists to analyze the behavior, mechanism, and relationship of individual cells.

In 2011, Nature Methods selected single-cell sequencing as one of the most anticipated technologies of the year. In 2013, Science ranked the single-cell sequence as the six most noteworthy areas of the year. At the top of the list, single-cell sequencing has become a research hotspot.

Evan Macosko of Harvard and MIT in the United States published an article on single-cell RNA sequencing, which received great attention. The detailed process is as follows:

Macosko et al., 2015, Cell 161, 1202–1214

The first author of the article, Macosko, recommended the use of the Dolomite chip from the United Kingdom for single-cell sequencing and further developed the system in cooperation with Dolomite.

Recommended chips are as follows: