An organelle in lysosomes in eukaryotic cells. In 1955, by the Belgian scholar C. R. De Diff et al. found in rat liver cells. Lysosome is a vesicular organelle surrounded by a single layer membrane containing various acidic hydrolases. Its main function is to carry out intracellular digestion and specialize in the decomposition of various exogenous and endogenous macromolecular substances. The lysosomes in the cells are heterogeneous, and the size and the type of hydrolase contained in the cells may be very different. The marker enzyme is acid phosphatase.
The current targeting of lysosomes is also mainly centered around acid phosphatase. classic 
LysoTracker series of lysosomal fluorescent dyes are widely used. Then, due to its cytotoxicity, LysoTracker loses its detection effect after several hours of staining, and it cannot continue to trace staining with cell passage. In addition, LysoTracker's easier to quench optical instability is often criticized by some researchers. Here, Aimeite recommends a series of lysosomal fluorescent staining kits consisting of LysoBrite's new generation of lysosomal-specific fluorescent dyes. As a new generation of fluorescent staining, LysoBrite has lower cytotoxicity, brighter fluorescence intensity, and higher fluorescence resistance to quenching!
The AAT Bioquest brand Cell Navigator lysosomal staining kit is designed to label mitochondria of living cells with different colors of fluorescence. This kit uses a proprietary dye that selectively accumulates in lysosomes. This LysoBrite lysosomal indicator is a hydrophobic complex that readily penetrates into living cells and is locked into the mitochondria when it enters the cell. This fluorescent lysosomal indicator can remain in the mitochondria for a long time because the indicator carries a group capable of localizing to the cell. Its key feature is its significant improvement in dyeing efficiency. The Cell Navigator lysosomal staining kit is very stable and requires very little hands-on. It can be used in many fluorescence studies such as microplate analysis, immunohistochemistry and flow cytometry. It can also be used in a variety of different types of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis, and cytotoxicity.
AAT Bioquest offers seven different color lysosomal fluorescence staining protocols that make it easy for customers to perform multi-color fluorescent labeling experiments, providing customers with great convenience. Available colors include blue 353/442nm, green 450/505 nm, orange 542/556 nm, red 575/597 nm, deep red 596/619 nm, and near-infrared 636/650 nm. To facilitate excitation and multicolor display in the UV, AAT Bioquest also offers a lysosomal staining kit that excites at 405 nm and emits green fluorescence at 505 nm. The kits offer all the essential components and optimal cell labeling methods for both proliferative and non-proliferating cells, as well as for suspension and adherent cells.
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| Blue/green/orange/red fluorescent stained lysosome | LysoBrite has higher fluorescence and quenching resistance than LysoTracker! |
Amy Jet also recommends AAT Bioquest's full line of Cell Navigator Fluorescence Kits, a powerful tool for labeling subcellular structures that mark cell membranes, lysosomes, mitochondria, nuclei, endoplasmic reticulum, and more. These selective dyes that subcellular structure of living cells provide researchers with a powerful weapon for studying cellular activity in a time and space context.
AAT Bioquest Inc. (formerly ABD Bioquest, Inc.) is a company that develops, manufactures and markets bioanalytical research reagents and kits for scientists engaged in life science research, diagnostic research and development, and drug development. The company is dedicated to the field of spectroscopy, including color development, fluorescence and bioluminescence. AAT Bioquest's products help scientists and biomedical researchers around the world better understand biochemistry, immunology, cell biology and molecular biology. As a regional agent for AAT Bioquest Inc. in China, Agilent Technologies provides Chinese customers with spectroscopy detection technology and products for biochemical, physiological metabolism and cell analysis, including color, fluorescence and luminescence technologies. As an agent for its well-known brands in China, Aimei can provide you with a range of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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