The importance of determining leaf area and measuring methods of photosynthesis in experiments

Why should I determine the leaf area?

Leaf area index is an important parameter for studying the material cycle, energy flow and plant productivity of ecosystems. As the most basic parameter to characterize vegetation canopy structure, it controls many biophysical processes of vegetation, such as photosynthesis, respiration, transpiration, carbon cycle and precipitation interception. For example, peanut photosynthetic area mainly refers to the area of ​​green leaves that can be used for photosynthesis. It is the most closely related, most varied and most controllable factor in photosynthesis, and more than 95% of dry matter is derived from photosynthesis of green leaf area.

Leaf area determination is the most important step in determining the leaf area index. The leaf area index is the ratio of the total area of ​​the leaves of a ground crop to the footprint. Namely: leaf area index = total area of ​​green leaves / floor area. The total area of ​​green leaves is calculated by measuring the area of ​​single leaves, the leaf area per plant, and then based on the number of crops per unit area of ​​land. Therefore, the leaf area is determined based on the leaf area index. The size of the leaf area directly affects the absorption of sunlight by the plant, which in turn affects the growth of the plant.

Measures such as rational close planting, balanced fertilization, timely irrigation, and rational use of plant growth regulators when planting peanuts are aimed at appropriately expanding and regulating leaf area. The leaf area is closely related to the light energy utilization and dry matter production of the peanut population. Therefore, rapid monitoring of peanut green leaf area in real time is of great significance for peanut growth diagnosis and management regulation and peanut yield prediction.

Because of the importance of leaf area measurement in agriculture, agricultural instrument companies have developed a variety of leaf area measuring instruments, including gps leaf area tester, timed positioning leaf area tester, handheld leaf area meter and so on. Using the leaf area meter to measure the leaf area, it is convenient to save trouble and quickly produce results. Then, by measuring the total area of ​​the land, the leaf area index can be calculated.

The whole process of photosynthesis can be expressed as:

It can be seen that the rate of consumption of any of the reactants or the rate of product formation (including exchange of materials and storage of energy) can be used to calculate the net photosynthetic rate (Pn). The corresponding net photosynthetic rate (Pn) can be roughly divided into:

(1) According to the rate of accumulation of organic matter, there are mainly half-leaf method and plant growth analysis method;

(2) According to the change of CO2 and O2 volume, there are mainly micro-definite pressure detection methods;

(3) According to the change of O2 concentration, the main aerobic electrode method;

(4) According to changes in CO2: acidity method, alkali absorption method, 14C labeling method, infrared gas analysis method, micro-meteorological method.

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